ENCBS437ZMP / cell line

Summary

Status
released
Term name
K562
Summary
Homo sapiens K562 cell line genetically modified (insertion) using CRISPR targeting H. sapiens ZNF324
Separated from biosample
ENCBS650NJZ
Parent of biosamples
ENCBS033CIS, ENCBS378SII

Attribution

ENCODE4 project
Lab
Michael Snyder, Stanford
Award PI
Michael Snyder, Stanford
Submitted by
Nathaniel Watson
Project
ENCODE
External resources
Aliases
michael-snyder:pB-3054, michael-snyder:pB-eGFP-ZNF324 K562

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM149CBTinsertiontaggingCRISPR

Donor information

Status
released
Accession
ENCDO000AAD
Aliases
encode:donor of K562, bradley-bernstein:Donor of K562 cells
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
female
Health status
chronic myelogenous leukemia (CML)
External resources
References

Documents

growth protocol
Description: Cell growth protocol for K562 cells (version 1).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
transfection protocol
Description excerpt: K562 transfection protocol - Version 1 (Replicates split at T-75 stage, 50 and…
Description
K562 transfection protocol - Version 1 (Replicates split at T-75 stage, 50 and 500 µg/ml G418)
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford