ENCBS418UGY / cell line

Summary

Status
released
Term name
MCF-7
Summary
Homo sapiens MCF-7 cell line stably expressing C-terminal eGFP-tagged KLF9
Description
MCF7 cell line stably expressing a C-terminal LAP-tag containing eGFP, fused to KLF9
Culture start date
2015-05-06
Culture harvest date
2015-07-13
Passage number
6

Attribution

ENCODE3 project
Lab
Kevin White, UChicago
Award PI
Michael Snyder, Stanford
Submitted by
Alec Victorsen
Project
ENCODE
Aliases
kevin-white:KLF9_GFP_MCF7_Rep3

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM189CRWinsertiontaggingstable transfection
Coordinates
hg19 chr9:72977475-73202397

Donor information

Status
released
Accession
ENCDO000AAE
Aliases
encode:donor of MCF-7, bradley-bernstein:Donor of MCF-7 cells
Species
Homo sapiens
Life stage
adult
Age
69 years
Sex
female
Health status
breast cancer (adenocarcinoma)
Ethnicity
Caucasian
External resources
References

Documents

immunoblot
Caption: immunoblot of GFP tagged KLF9, expressed in MCF7 cells
Attachment from submitter
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
immunofluorescence
Caption: immunofluorescence of GFP tagged KLF9 in MCF7 cells
Attachment from submitter
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
general protocol
Description: Protocol for generating GFP tagged BAC transgenic lines in MCF7.
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago