ENCBS386FXR / cell line
Summary
- Status
- released
- Term name
- K562
- Term ID
- EFO:0002067
- Summary
- Homo sapiens K562 cell line genetically modified (insertion) using transduction (low MOI), genetically modified using CRISPR cutting (sgRNA) 10 days after the sample was treated with Puromycin
- Culture start date
- 2020-10-25
- Culture harvest date
- 2020-11-11
- Passage number
- 9
Attribution
ENCODE4 project
- Lab
- Yin Shen, UCSF
- Award PI
- Yin Shen, UCSF
- Submitted by
- Lazaros Lataniotis
- Source
- ATCC
- Project
- ENCODE
- External resources
- Aliases
- yin-shen:DCC_K562_MYC_GFP_MYC_Screening_FACS_99-100perCent_Biosample_Rep2
Treatment details
- Treatment
- Puromycin (CHEBI:17939)
- Type
- chemical
- Purpose
- selection
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM063WJB | CRISPR cutting | characterization | CRISPR | ||
| ENCGM885GEZ | insertion | expression | transduction |
Donor information
- Status
- released
- Accession
- ENCDO000AAD
- Aliases
- encode:donor of K562, bradley-bernstein:Donor of K562 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- female
- Health status
- chronic myelogenous leukemia (CML)
- External resources
- References