ENCBS385UEE / cell line

Summary

Status
released
Term name
WTC11
Summary
Homo sapiens WTC11 cell line genetically modified (insertion) using transduction, genetically modified (interference) using CRISPR treated with 160 ng/mL tioguanine for 7 days
Culture start date
2018-11-08
Culture harvest date
2018-12-03
Passage number
48
Separated from biosample
ENCBS000ECX

Attribution

ENCODE4 project
Lab
Yin Shen, UCSF
Award PI
Yin Shen, UCSF
Submitted by
Xingjie Ren
Project
ENCODE
External resources
Aliases
yin-shen:WTC11_CRISPRi_2_2X6TG

Treatment details

Treatment
160 ng/mL tioguanine (CHEBI:9555) for 7 days
Type
chemical

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM361RWUinterferencecharacterizationCRISPR
ENCGM171IJGinsertionexpressiontransduction

Donor information

Status
released
Accession
ENCDO882UJI
Aliases
encode:donor of WTC11
Species
Homo sapiens
Life stage
adult
Age
30 years
Sex
male
Ethnicity
Japanese