ENCBS335UYM / whole organisms
Summary
- Status
- released
- Term name
- whole organism
- Term ID
- UBERON:0000468
- Summary
- Drosophila melanogaster strain Hr3-GFP embryo (0-8 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Hr3
- Life stage
- embryonic
- Age
- 0-8 hours
Attribution
modENCODE Project
- Lab
- Kevin White, UChicago
- Award PI
- Kevin White, UChicago
- Submitted by
- Kevin White
- Source
- Kevin White
- Project
- modENCODE
- Aliases
- kevin-white:B1-modencode_5023
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM719SOC | insertion | tagging | site-specific recombination |
|
Strain information
- Status
- released
- Accession
- ENCDO395MOB
- Aliases
- modencode:Hr3-GFP, bloomington:42274, modencode:Hr46-GFP
- Donor external identifiers
- Species
- Drosophila melanogaster
- Genotype
- w[1118]; PBac{y[+mDint2] w[+mC]=Hr3-GFP.FLAG}VK00033/TM3, Sb[1]
- Sex
- unknown
- Strain background
- VK00033
- Strain name
- Hr3-GFP
- External resources
Documents
immunofluorescence
Caption excerpt: Strain name: Hr46-GFP
Genotype: PBac{y[+]-attP-3B}VK00033
Gene…
- Caption
- Strain name: Hr46-GFP Genotype: PBac{y[+]-attP-3B}VK00033 Gene Target: Hr46 Transgene: Hr46 genomic coding region, stop codon replaced with GFP Tag Type: LAP-TAG_EGFP Short Description: P[acman] BAC number CH321-23L02 has been chosen to target the gene Hormone receptor-like in 46. The LAP-TAG containing the GFP has been recombineered in 3' of the gene in frame. The engineered vector has then been inserted into the specific docking site with the help of Att sites. Recombinant individuals were screened based on mini-white presence. Vector Used: P[acman] Genomic Coordinates: 2R from 6079816 to 6166290. attP Coordinates: 3L:6435776..6435776 Made_by: Alec Victorsen, Rebecca Spokony
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U01HG004264
immunofluorescence
Caption excerpt: Strain name: Hr46-GFP
Genotype: PBac{y[+]-attP-3B}VK00033
Gene…
- Caption
- Strain name: Hr46-GFP Genotype: PBac{y[+]-attP-3B}VK00033 Gene Target: Hr46 Transgene: Hr46 genomic coding region, stop codon replaced with GFP Tag Type: LAP-TAG_EGFP Short Description: P[acman] BAC number CH321-23L02 has been chosen to target the gene Hormone receptor-like in 46. The LAP-TAG containing the GFP has been recombineered in 3' of the gene in frame. The engineered vector has then been inserted into the specific docking site with the help of Att sites. Recombinant individuals were screened based on mini-white presence. Vector Used: P[acman] Genomic Coordinates: 2R from 6079816 to 6166290. attP Coordinates: 3L:6435776..6435776 Made_by: Alec Victorsen, Rebecca Spokony
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U01HG004264