ENCBS333TCO / cell line
Summary
- Status
- released
- Term name
- K562
- Term ID
- EFO:0002067
- Summary
- Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL Puromycin for 96 hours, 21 days post-nucleic acid delivery time
- Post-nucleic acid delivery time
- 21 days
Attribution
ENCODE4 project
- Lab
- Will Greenleaf, Stanford
- Award PI
- Will Greenleaf, Stanford
- Submitted by
- David Yao
- Source
- Michael Bassik, Stanford
- Project
- ENCODE
- External resources
- Aliases
- will-greenleaf:K562_LargeScale_DHS_Growth_dCas9-KRAB-MGA1-MGA2_T21_R2.Biosample
Treatment details
- Treatment
- 1 μg/mL Puromycin (CHEBI:17939) for 96 hours
- Type
- chemical
- Purpose
- selection
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM484HMC | insertion | expression | transduction | ||
| ENCGM149QCD | CRISPRi | characterization | CRISPR | transduction |
Donor information
- Status
- released
- Accession
- ENCDO000AAD
- Aliases
- encode:donor of K562, bradley-bernstein:Donor of K562 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- female
- Health status
- chronic myelogenous leukemia (CML)
- External resources
- References