ENCBS311LDY / primary cell
Summary
- Status
- released
- Term name
- natural killer cell
- Term ID
- CL:0000623
- Summary
- Homo sapiens male adult (47 years) natural killer primary cell treated with 100 ng/mL Interleukin-12 subunit alpha for 72 hours, 100 ng/mL Interleukin-15 for 72 hours, 100 ng/mL Interleukin-12 subunit beta for 72 hours, 100 ng/mL Interleukin-18 for 72 hours
- Separated from biosample
- ENCBS962ZOS
Attribution
ENCODE4 project
- Lab
- John Stamatoyannopoulos, UW
- Award PI
- John Stamatoyannopoulos, UW
- Submitted by
- Michael Buckley
- Source
- StemExpress
- Project
- ENCODE
- External resources
- Aliases
- john-stamatoyannopoulos:TC18517
- Submitter comment
- DONOR 2: 72 hour activation IL-12+IL-15+IL-18, 100ng/ml
Treatment details
- Treatment
- 100 ng/mL Interleukin-12 subunit alpha (UniProtKB:P29459) for 72 hours
- Type
- protein
- Purpose
- stimulation
- Treatment
- 100 ng/mL Interleukin-15 (UniProtKB:P40933) for 72 hours
- Type
- protein
- Purpose
- stimulation
- Treatment
- 100 ng/mL Interleukin-12 subunit beta (UniProtKB:P29460) for 72 hours
- Type
- protein
- Purpose
- stimulation
- Treatment
- 100 ng/mL Interleukin-18 (UniProtKB:Q14116) for 72 hours
- Type
- protein
- Purpose
- stimulation
Donor information
- Status
- released
- Accession
- ENCDO311UBW
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 47 years
- Sex
- male
- External resources
- Submitter comment
- StemExpress:RD01000364
Documents
cell isolation protocol
Description excerpt: Isolation of Immune cells by Stemcell Technologies Magnetic Beads kits and…
- Description
- Isolation of Immune cells by Stemcell Technologies Magnetic Beads kits and stimulations (Stamatoyannopoulos lab). This protocol is an updated version of : https://www.encodeproject.org/documents/b94d1a22-1ef2-4820-9b71-cdcfba41127c/
- Submitted by
- Jennifer Jou
- Lab
- John Stamatoyannopoulos, UW
- Grant
- UM1HG009444