ENCBS239UQV / cell line
Summary
- Status
- released
- Term name
- BJAB
- Term ID
- EFO:0002815
- Summary
- Homo sapiens BJAB cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 4 μg/mL anti-CD40 for 4 hours, 10 μg/mL anti-IgM for 4 hours
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award PI
- Will Greenleaf, Stanford
- Submitted by
- Benjamin Doughty
- Source
- ATCC
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-BJAB-True-PPIF-biorep2-binB_biosamp
Treatment details
- Treatment
- 4 μg/mL anti-CD40 for 4 hours
- Type
- antibody
- Purpose
- stimulation
- Treatment
- 10 μg/mL anti-IgM for 4 hours
- Type
- antibody
- Purpose
- activation
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM100KJF | CRISPRi | characterization | CRISPR | ||
| ENCGM324JGL | insertion | expression | transduction |
Donor information
- Status
- released
- Accession
- ENCDO868QMD
- Aliases
- encode:bjab-donor
- Species
- Homo sapiens
- Life stage
- child
- Age
- 5 years
- Sex
- female
- Ethnicity
- Black African