ENCBS222ZSB / primary cell

Summary

Status
released
Term name
activated T-helper 17 cell
Term ID
NTR:0000635
Summary
Homo sapiens male adult (48 years) activated T-helper 17 primary cell treated with 50 U/mL Interleukin-2 for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours
Separated from biosample
ENCBS718CSV

Attribution

ENCODE4 project
Lab
John Stamatoyannopoulos, UW
Award PI
John Stamatoyannopoulos, UW
Submitted by
Michael Buckley
Project
ENCODE
External resources
Aliases
john-stamatoyannopoulos:TC18651
Submitter comment
DONOR 5: 72 Hour T cell activation (CD3.CD28 beads) + 50U/ml IL-2

Treatment details

Treatment
50 U/mL Interleukin-2 (UniProtKB:P60568) for 72 hours
Type
protein
Purpose
activation
Treatment
anti-CD3 and anti-CD28 coated beads for 72 hours
Type
antibody
Purpose
activation

Donor information

Status
released
Accession
ENCDO662KZD
Species
Homo sapiens
Life stage
adult
Age
48 years
Sex
male
External resources
Submitter comment
StemExpress:RD01000670

Documents

cell isolation protocol
Description excerpt: Isolation of Immune cells by Stemcell Technologies Magnetic Beads kits and…
Description
Isolation of Immune cells by Stemcell Technologies Magnetic Beads kits and stimulations (Stamatoyannopoulos lab). This protocol is an updated version of : https://www.encodeproject.org/documents/b94d1a22-1ef2-4820-9b71-cdcfba41127c/
Submitted by
Jennifer Jou
Lab
John Stamatoyannopoulos, UW