ENCBS218KXX / primary cell
Summary
- Status
- released
- Term name
- stimulated activated naive B cell
- Term ID
- NTR:0000661
- Summary
- Homo sapiens female adult (39 years) stimulated activated naive B primary cell treated with 1 μg/mL anti-CD40 for 72 hours, 100 ng/mL Interleukin-4 for 72 hours, 10 μg/mL anti-IgM for 72 hours
- Culture start date
- 2021-06-07
- Culture harvest date
- 2021-06-22
- Separated from biosample
- ENCBS234ZWK
Attribution
ENCODE4 project
- Lab
- Bradley Bernstein, Broad
- Award PI
- Bradley Bernstein, Broad
- Submitted by
- Siddarth Wekhande
- Source
- John Stamatoyannopoulos
- Project
- ENCODE
- External resources
- Aliases
- bradley-bernstein:BioSam 4526
Treatment details
- Treatment
- 1 μg/mL anti-CD40 for 72 hours
- Type
- antibody
- Purpose
- activation
- Treatment
- 100 ng/mL Interleukin-4 (UniProtKB:P05112) for 72 hours
- Type
- protein
- Purpose
- stimulation
- Treatment
- 10 μg/mL anti-IgM for 72 hours
- Type
- antibody
- Purpose
- activation
Donor information
- Status
- released
- Accession
- ENCDO352FHC
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 39 years
- Sex
- female
- External resources
- Submitter comment
- StemExpress:B001001875
Documents
cell isolation protocol
Description excerpt: Isolation of Immune cells by Stemcell Technologies Magnetic Beads kits and…
- Description
- Isolation of Immune cells by Stemcell Technologies Magnetic Beads kits and stimulations (Stamatoyannopoulos lab). This protocol is an updated version of : https://www.encodeproject.org/documents/b94d1a22-1ef2-4820-9b71-cdcfba41127c/
- Submitted by
- Jennifer Jou
- Lab
- John Stamatoyannopoulos, UW
- Grant
- UM1HG009444