ENCBS189BWL / cell line
Summary
- Status
- released
- Term name
- K562
- Term ID
- EFO:0002067
- Summary
- Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting H. sapiens EIF4A3
- Description
- RNA-seq on K562 cells treated with a CRISPR gRNA against EIF4A3. (EIF4A3-BGKcLV15-32)
- Product ID
- CCL-243
- Lot ID
- 59300853
- Culture start date
- 2019-03-26
- Culture harvest date
- 2019-04-08
Attribution
ENCODE4 project
- Lab
- Brenton Graveley, UConn
- Award PI
- Brenton Graveley, UConn
- Submitted by
- Xintao Wei
- Source
- ATCC
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:EIF4A3-BGKcLV15-32
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM715JKM | deletion | repression | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAD
- Aliases
- encode:donor of K562, bradley-bernstein:Donor of K562 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- female
- Health status
- chronic myelogenous leukemia (CML)
- External resources
- References
Documents
general protocol
Description: Biosample protocol for sample EIF4A3-BGKcLV15-32
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889
immunoblot
Caption excerpt: Western blot following CRISPR against EIF4A3 in K562 whole cell lysate using…
- Caption
- Western blot following CRISPR against EIF4A3 in K562 whole cell lysate using EIF4A3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4A3. EIF4A3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889