ENCBS180COF / cell line
Summary
- Status
- released
- Term name
- K562
- Term ID
- EFO:0002067
- Summary
- Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA), 21 days post-nucleic acid delivery time
- Description
- CRISPRi perturbation of K562s. K562 leukemia cells engineered to express dCas9-KRAB transduced with an 18-sgRNA lentiviral pool, 21 days post-transduction
- Post-nucleic acid delivery time
- 21 days
Attribution
ENCODE4 project
- Lab
- Will Greenleaf, Stanford
- Award PI
- Will Greenleaf, Stanford
- Submitted by
- Tao Wang
- Source
- ATCC
- Project
- ENCODE
- External resources
- Aliases
- will-greenleaf:sp_biosample5
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM795IKY | CRISPRi | characterization | CRISPR | ||
| ENCGM617AEN | insertion | expression | transduction |
Donor information
- Status
- released
- Accession
- ENCDO000AAD
- Aliases
- encode:donor of K562, bradley-bernstein:Donor of K562 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- female
- Health status
- chronic myelogenous leukemia (CML)
- External resources
- References