ENCBS164UBJ / cell line

Summary

Status
released
Term name
K562
Summary
Homo sapiens K562 cell line stably expressing C-terminal eGFP-tagged GABPA
Description
K562 cell line stably expressing a C-terminal LAP-tag containing eGFP, fused to GABPA
Culture start date
2015-08-27
Culture harvest date
2015-10-23
Passage number
4

Attribution

ENCODE3 project
Lab
Kevin White, UChicago
Award PI
Michael Snyder, Stanford
Submitted by
Alec Victorsen
Project
ENCODE
Aliases
kevin-white:GABPA_GFP_K562_INPUT

Genetic modifications

Accession
Category
Purpose
Method
Site
ENCGM292VONinsertiontaggingstable transfection
Coordinates
hg19 chr21:26958607-27169245

Donor information

Status
released
Accession
ENCDO000AAD
Aliases
encode:donor of K562, bradley-bernstein:Donor of K562 cells
Donor external identifiers
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
female
Health status
chronic myelogenous leukemia (CML)
References

Documents

immunoblot
Caption: immunoblot of GFP tagged GABPA, expressed in K562 cells
Attachment from submitter
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
FACs analysis
Caption excerpt: eGFP-GABPA tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby…
Attachment from submitter
Caption
eGFP-GABPA tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP+ fraction quantified for their GFP and nuclear signal colocalization.
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
FACs analysis
Caption excerpt: eGFP-GABPA tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby…
Attachment from submitter
Caption
eGFP-GABPA tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP++ fraction quantified for their GFP and nuclear signal colocalization.
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
FACs analysis
Caption excerpt: eGFP-GABPA tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby…
Attachment from submitter
Caption
eGFP-GABPA tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP+ fraction quantified for their GFP and nuclear signal colocalization.
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
general protocol
Description: Protocol for generating GFP tagged BAC transgenic lines in K562.
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago