ENCBS148PXX / primary cell
Summary
- Status
- released
- Term name
- activated T-cell
- Term ID
- NTR:0000537
- Summary
- Homo sapiens male adult (43 years) activated T-cell primary cell treated with 50 U/mL Interleukin-2 for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours
- Separated from biosample
- ENCBS489OLX
Attribution
ENCODE4 project
- Lab
- John Stamatoyannopoulos, UW
- Award PI
- John Stamatoyannopoulos, UW
- Submitted by
- Michael Buckley
- Source
- StemExpress
- Project
- ENCODE
- Aliases
- john-stamatoyannopoulos:TC18507
- Submitter comment
- DONOR 1: 72 Hour T cell activation (CD3.CD28 beads) + 50U/ml IL-2
Treatment details
- Treatment
- 50 U/mL Interleukin-2 (UniProtKB:P60568) for 72 hours
- Type
- protein
- Purpose
- activation
- Treatment
- anti-CD3 and anti-CD28 coated beads for 72 hours
- Type
- antibody
- Purpose
- activation
Donor information
- Status
- released
- Accession
- ENCDO106CDP
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 43 years
- Sex
- male
- External resources
- Submitter comment
- StemExpress:D001005998
Documents
cell isolation protocol
Description excerpt: Isolation of Immune cells by Stemcell Technologies Magnetic Beads kits and…
- Description
- Isolation of Immune cells by Stemcell Technologies Magnetic Beads kits and stimulations (Stamatoyannopoulos lab). This protocol is an updated version of : https://www.encodeproject.org/documents/b94d1a22-1ef2-4820-9b71-cdcfba41127c/
- Submitted by
- Jennifer Jou
- Lab
- John Stamatoyannopoulos, UW
- Grant
- UM1HG009444