ENCBS131RZN / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Drosophila melanogaster strain 10T-GFP embryo (0-8 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Dfd
Life stage
embryonic
Age
0-8 hours

Attribution

modENCODE Project
Lab
Kevin White, UChicago
Award PI
Kevin White, UChicago
Submitted by
Kevin White
Project
modENCODE
Aliases
kevin-white:B2-modencode_4075

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM734VBSinsertiontaggingsite-specific recombination
Coordinates
dm3 chr2L:17364165-17471548

Strain information

Status
released
Accession
ENCDO202SKL
Aliases
bloomington:30877
Donor external identifiers
Species
Drosophila melanogaster
Genotype
y[1] w[*]; PBac{y[+mDint2] w[+mC]=Dfd-EGFP.S}VK00037
Sex
unknown
Strain background
VK00037
Strain name
10T-GFP

Documents

construct image
Description excerpt: The LAP tag contains TEV and PreScission protease cleavage sites, s-peptide…
Attachment from submitter
Description
The LAP tag contains TEV and PreScission protease cleavage sites, s-peptide sequence, eGFP, an internal ribosomal biding site and a bacterial promoter driving a Kan/Neo cassette.
Submitted by
Mark Murphy
Lab
Kevin White, UChicago
construct image
Description: P[acman] vector map retreived from bacpac.chori.org on 7-10-2014
Attachment from submitter
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago