ENCBS086JQA / cell line

Summary

Status
released
Term name
K562
Summary
Homo sapiens K562 cell line expressing RNAi
Description
K562 cells treated with a control shRNA against no target, used in RNA-seq experiments. (k562-shC-1)
Product ID
CCL-243
Lot ID
59300853
Culture harvest date
2013-05-25

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award PI
Brenton Graveley, UConn
Submitted by
Xintao Wei
Source
ATCC
Project
ENCODE
Aliases
brenton-graveley:k562-shC-1, brenton-graveley:BGK0016B-1
Tags
Search for all Biosample with internal tag ENCORE

Genetic modifications

Accession
Category
Purpose
Method
Site
ENCGM266AWPinterferencenon-specific target controlRNAi

Donor information

Status
released
Accession
ENCDO000AAD
Aliases
encode:donor of K562, bradley-bernstein:Donor of K562 cells
Donor external identifiers
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
female
Health status
chronic myelogenous leukemia (CML)
References

Documents

growth protocol
Description: Growth protocol for K562 cells used in the RNA evaluation dataset for ENCODE
Submitted by
Brenton Graveley
Lab
Brenton Graveley, UConn
transfection protocol
Description: Transfection protocol for shRNA transfection in K562 cells
Submitted by
Brenton Graveley
Lab
Brenton Graveley, UConn