ENCBS069IQK / primary cell

Summary

Status
released
Term name
activated T-helper 17 cell
Term ID
NTR:0000635
Summary
Mus musculus strain C57BL/6NJ activated T-helper 17 primary cell genetically modified (episome) using transient transfection treated with 0.25 μg/mL anti-CD3e for 3 days, 1 μg/mL anti-CD28 for 3 days, 2 μg/mL anti-Interleukin-4 for 3 days, 2 μg/mL anti-Interferon-gamma for 3 days, 10 ng/mL Interleukin-6 for 3 days, 0.3 ng/mL Transforming growth factor beta-1 proprotein for 3 days
Life stage
unknown
Age
Unknown

Attribution

ENCODE4 project
Lab
Tim Reddy, Duke
Award PI
Tim Reddy, Duke
Submitted by
Revathy Venukuttan
Project
ENCODE
Aliases
tim-reddy:Th17-atac-starrseq-biosample-rep2

Treatment details

Treatment
0.25 μg/mL anti-CD3e for 3 days
Type
antibody
Purpose
activation
Treatment
1 μg/mL anti-CD28 for 3 days
Type
antibody
Purpose
activation
Treatment
2 μg/mL anti-Interleukin-4 for 3 days
Type
antibody
Purpose
activation
Treatment
2 μg/mL anti-Interferon-gamma for 3 days
Type
antibody
Purpose
activation
Treatment
10 ng/mL Interleukin-6 (UniProtKB:P08505) for 3 days
Type
protein
Purpose
activation
Treatment
0.3 ng/mL Transforming growth factor beta-1 proprotein (UniProtKB:P01137) for 3 days
Type
protein
Purpose
activation

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM741TNPepisomecharacterizationtransient transfection

Strain information

Status
released
Accession
ENCDO236IKC
Aliases
encode:C57BL_6NJ
Donor external identifiers
Species
Mus musculus
Sex
unknown
Strain background
C57BL/6
Strain name
C57BL/6NJ