ENCBS049MVX / cell line
Summary
- Status
- released
- Term name
- HCT116
- Term ID
- EFO:0002824
- Summary
- Homo sapiens HCT116 cell line genetically modified (insertion) using CRISPR inserting O. sativa LOC4335696, genetically modified (insertion) using CRISPR targeting H. sapiens RAD21 treated with 87.6 μg/mL indole-3-acetic acid for 6 hours
- Description
- in situ Hi-C of human cell line: HCT-116-RAD21-mAC cells, 6 hours of auxin treatment
- Starting amount
- 20000000cells
- Culture start date
- 2016-11-03
- Culture harvest date
- 2016-11-08
Attribution
ENCODE4 project
- Lab
- Erez Aiden, Baylor
- Award PI
- Erez Aiden, Baylor
- Submitted by
- Neva Durand
- Source
- Dr. Masato Kanemaki
- Project
- ENCODE
- External resources
- Aliases
- erez-aiden:HCT-116-RAD21-mAC-6hr-auxin-biorep2
Treatment details
- Treatment
- 87.6 μg/mL indole-3-acetic acid (CHEBI:16411) for 6 hours
- Type
- chemical
- Purpose
- perturbation
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
---|---|---|---|---|---|
ENCGM785YNS | insertion | tagging | CRISPR |
| |
ENCGM489TXA | insertion | expression | CRISPR | ||
ENCGM561PJN | insertion | tagging | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000ABE
- Aliases
- encode:donor of HCT-116, bradley-bernstein:Donor of HCT-116 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 48 years
- Sex
- male
- Health status
- colorectal carcinoma
- External resources
- References