ENCBS035NHH / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain YL478 hermaphrodite young adult whole organism genetically modified (insertion) using bombardment targeting efl-1
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to EFL-1
Life stage
young adult
Age
Unknown

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
Aliases
valerie-reinke:EFL-1_GFP_YL478_yAd_Rep1

Genetic modifications

Accession
Category
Purpose
Method
Site
ENCGM506WFHinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO660UNK
Aliases
valerie-reinke:YL478
Species
Caenorhabditis elegans
Sex
hermaphrodite
Strain name
YL478

Documents

general protocol
Description: Protocol used by the Reinke lab for ChIP-seq
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale
immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale