ENCBS017YHL / cell line
Summary
- Status
- released
- Term name
- Jurkat
- Term ID
- EFO:0002796
- Summary
- Homo sapiens Jurkat cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 5 μg/mL anti-CD3 for 4 hours, 100 ng/mL phorbol 12-myristate 13-acetate for 4 hours
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award PI
- Will Greenleaf, Stanford
- Submitted by
- Benjamin Doughty
- Source
- ATCC
- Project
- ENCODE
- External resources
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-Jurkat-True-PPIF-biorep1-binF_biosamp
Treatment details
- Treatment
- 5 μg/mL anti-CD3 for 4 hours
- Type
- antibody
- Purpose
- stimulation
- Treatment
- 100 ng/mL phorbol 12-myristate 13-acetate (CHEBI:37537) for 4 hours
- Type
- chemical
- Purpose
- stimulation
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM212EML | CRISPRi | characterization | CRISPR | ||
| ENCGM324JGL | insertion | expression | transduction |
Donor information
- Status
- released
- Accession
- ENCDO000AAY
- Aliases
- encode:donor of Jurkat, encode:donor of Jurkat clone E6-1
- Species
- Homo sapiens
- Life stage
- child
- Age
- 14 years
- Sex
- male
- Health status
- acute T cell leukemia
- External resources