ENCBS002VIT / cell line
Summary
- Status
- released
- Term name
- K562
- Term ID
- EFO:0002067
- Summary
- Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting H. sapiens DCP1A
- Description
- RNA-seq on K562 cells treated with a CRISPR gRNA against DCP1A. (DCP1A-BGKcLV28-61)
- Product ID
- CCL-243
- Lot ID
- 59300853
- Culture start date
- 2021-06-04
- Culture harvest date
- 2021-06-16
Attribution
ENCODE4 project
- Lab
- Brenton Graveley, UConn
- Award PI
- Brenton Graveley, UConn
- Submitted by
- Xintao Wei
- Source
- ATCC
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:DCP1A-BGKcLV28-61
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM932QDC | deletion | repression | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAD
- Aliases
- encode:donor of K562, bradley-bernstein:Donor of K562 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- female
- Health status
- chronic myelogenous leukemia (CML)
- External resources
- References
Documents
general protocol
Description: Biosample protocol for sample DCP1A-BGKcLV28-61
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889
immunoblot
Caption excerpt: Western blot following CRISPR against DCP1A in K562 whole cell lysate using…
- Caption
- Western blot following CRISPR against DCP1A in K562 whole cell lysate using DCP1A specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DCP1A. DCP1A protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889