1. Antibodies
  2. Homo sapiens
  3. CHAMP1

ENCAB986MDC

Antibody against Homo sapiens CHAMP1

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A304-216A
Lot ID
1
Characterized targets
CHAMP1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1294
External resources

Characterizations

CHAMP1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A304-216A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected Molecular Weight: 90 KD
Reviewer comment
This seems a bit on the heavy side
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
CHAMP1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A304-216A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 89.099.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
CHAMP1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A304-216A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
Both Genecard and Biogrid reported that POGZ and CHAMP1 has interactions. http://www.genecards.org/cgi-bin/carddisp.pl?gene=CHAMP1&keywords=champ1. http://thebiogrid.org/129584/summary/homo-sapiens/champ1.html
Reviewer comment
Not the highest ranked factor but higher ranked TF (POGZ) is a known interactor.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford