ENCAB975FNJ
Antibody against Homo sapiens WDR5
Homo sapiens
K562, HepG2, GM12878, endothelial cell of umbilical vein
not characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A302-429A
- Lot ID
- 2
- Characterized targets
- WDR5 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Aliases
- bradley-bernstein:PchAb 1270
- External resources
Characterizations
WDR5 (Homo sapiens)
K562HepG2GM12878GM12878
not compliant
- Caption
- 2g nuclear lystate for each IP. 2ug of primary antibody and 50uL of pA/G beads. Used a 4-12% Bis-Tris gel in 1X MOPS running buffer. iBlot transfer, Program 3, 6 minutes. Blocked overnight at 4'C in TBS-T with 5% BSA. Antibodies in TBS-T with 5% BSA. Primary antibody overnight at 4'C. Secondary antibody used for 1 hour at RT. 0.75 second exposure in ChemiDoc. Expected band size at 36kDa.
- Reviewer comment
- KCO: expected band isn't >50% of all bands in its given lane.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- WDR5_Bethyl_A302-429A-2_IPAS.png
WDR5 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
not compliant
- Caption
- Nuclear lysates from K562 (10ug), HepG2 (10 ug), GM12878 (10ug), and HUVEC (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. A range of bands were detected outside of the expected size (36kDa).
- Reviewer comment
- KCO: expected band isn't >50% of all bands in its given lane.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- WDR5_Bethyl_A302-429A-2_WB.png