ENCAB943WPC
Antibody against Homo sapiens H3K27ac, Mus musculus H3K27ac
Mus musculus
any cell type or tissue
characterized to standards
Homo sapiens
any cell type or tissue
characterized to standards
- Status
- released
- Source (vendor)
- Active Motif
- Product ID
- 39133
- Lot ID
- 16612005
- Characterized targets
- H3K27ac (Homo sapiens), H3K27ac (Mus musculus)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- Protein A
- Isotype
- IgG
- Antigen description
- Histone H3 acetyl Lys27 antibody was raised against a peptide including acetyl-lysine 27 of histone H3
- Aliases
- bing-ren:4.6
- External resources
Characterizations
H3K27ac (Homo sapiens)
compliant
- Caption
- Performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-668, http://www.millipore.com/catalogue/item/16-668#). The top panel contains histone H2A, H2B, and H4 peptides while the bottom panel has histone H3 peptides. The antibody recognized the H3K27ac peptide 100% (noted with red #1).
- Submitted by
- Ah Young Lee
- Lab
- Bing Ren, UCSD
- Grant
- U54HG006997
- Download
- bing-ren_4.6_dotblot.jpeg
H3K27ac (Mus musculus)
compliant
- Caption
- Performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-668, http://www.millipore.com/catalogue/item/16-668#). The top panel contains histone H2A, H2B, and H4 peptides while the bottom panel has histone H3 peptides. The antibody recognized the H3K27ac peptide 100% (noted with red #1).
- Submitted by
- Ah Young Lee
- Lab
- Bing Ren, UCSD
- Grant
- U54HG006997
- Download
- bing-ren_4.6_dotblot.jpeg
H3K27ac (Mus musculus)
embryonic fibroblastTT2
compliant
- Caption
- An immunoblot using whole cell extract of two human cell lines and two mouse cell lines shows positive bands at the size of the histone (17kDa), while the recombinant unmodified version of that histone shows a lack of reactivity. The histone bands detected in the extracts constitute 100% of the protein signal and show at least 10-fold enrichment relative to any other band. This signal is also at least 10-fold enriched relative to that detected using the unmodified recombinant histone.
- Submitted by
- Ah Young Lee
- Lab
- Bing Ren, UCSD
- Grant
- U54HG006997
- Download
- bing-ren_4.6_Western.jpg
H3K27ac (Homo sapiens)
A375HEK293
compliant
- Caption
- An immunoblot using whole cell extract of two human cell lines and two mouse cell lines shows positive bands at the size of the histone (17kDa), while the recombinant unmodified version of that histone shows a lack of reactivity. The histone bands detected in the extracts constitute 100% of the protein signal and show at least 10-fold enrichment relative to any other band. This signal is also at least 10-fold enriched relative to that detected using the unmodified recombinant histone.
- Submitted by
- Ah Young Lee
- Lab
- Bing Ren, UCSD
- Grant
- U54HG006997
- Download
- bing-ren_4.6_Western.jpg