1. Antibodies
  2. Homo sapiens
  3. IGF2BP3

ENCAB934MDN

Antibody against Homo sapiens IGF2BP3

Homo sapiens
HepG2, K562
characterized to standards
Status
released
Source (vendor)
MBLI
Product ID
RN009P
Lot ID
002
Characterized targets
IGF2BP3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
IgA
Antigen description
KLH-conjugated synthetic peptide HQQQKALQSGPPQSRRK (563-579 aa)
External resources

Characterizations

IGF2BP3 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using IGF2BP3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-IGF2BP3 antibody (lanes under 'IGF2BP3').
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
IGF2BP3 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following shRNA against IGF2BP3 in K562 and HepG2 whole cell lysate using IGF2BP3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against IGF2BP3. Lanes follow the same pattern, but in HepG2. IGF2BP3 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
IGF2BP3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using IGF2BP3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lanes 4 and 5 are 10% IP enrichments of biological replicates using rabbit polyclonal anti-IGF2BP3 antibody (lanes under 'IGF2BP3').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD