ENCAB904KUI
Antibody against Homo sapiens REST
Homo sapiens
K562, HEK293T
characterized to standards
Homo sapiens
MCF-7
not characterized to standards
- Status
- released
- Source (vendor)
- Sigma
- Product ID
- HPA006079
- Lot ID
- B69614
- Characterized targets
- REST (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- RE1-silencing transcription factor recombinant protein epitope signature tag (PrEST)
- Antigen sequence
- SPPLPKENLREEASGDQKLLNTGEGNKEAPLQKVGAEEADESLPGLAANINESTHISSSGQNLNTPEGETLNGKHQTDSIVCEMKMDTDQNTRENLTGINSTVEEPVSPMLPPSAVEEREAVSKTALA
- Aliases
- michael-snyder:696
- External resources
Characterizations
REST (Homo sapiens)
MCF-7
Method: immunoprecipitation
not compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody HPA006079. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Reviewer comment
- Not within 20% of expected size.
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
REST (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody HPA006079. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected Molecular Weight: 122 KD
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 3_REST.jpg
REST (Homo sapiens)
Method: motif enrichment
compliant
- Caption
- The motif for target REST is represented by the attached position weight matrix (PWM) derived from ENCFF603TZF. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.957933587; Global enrichment Z-score: 6.241552865; Positional bias Z-score: 6.448790114; Peak rank bias Z-score: 11.55462638; Enrichment rank: 1.0.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
REST (Homo sapiens)
HEK293T
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody HPA006079. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 121.872
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- Expt1123_6-REST-HPA006079.JPG