ENCAB872RJV
Antibody against Homo sapiens DEPDC1B
Homo sapiens
K562, GM12878
partially characterized
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A302-517A
- Lot ID
- 1
- Characterized targets
- DEPDC1B (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Aliases
- bradley-bernstein:PchAb 1299
- External resources
Characterizations
DEPDC1B (Homo sapiens)
K562GM12878
compliant
- Caption
- Nuclear lysates from K562 (10ug) and GM12878 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MESS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected at the expected size (36kDa) and outside of the expected range as well. **Molecular mass: 61771 Da**
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- BRCC3_BRCC36_Bethyl_A302-517A-1.png