ENCAB864VOP
Antibody against Homo sapiens EIF4E
Homo sapiens
K562
characterized to standards
- Status
- released
- Source (vendor)
- Thermo Fisher
- Product ID
- MA1-089
- Lot ID
- TD257774
- Characterized targets
- EIF4E (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Purification
- Protein A
- Isotype
- IgG1
- Antigen description
- Full length human eIF4E produced in HEK293T cells
- External resources
Characterizations
EIF4E (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against EIF4E in HepG2 whole cell lysate using EIF4E specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4E. EIF4E protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889
- Download
- EIF4E-HEPG2-CRISPR-MA1-089.png
EIF4E (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against EIF4E in K562 whole cell lysate using EIF4E specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4E. EIF4E protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889
- Download
- EIF4E-K562-CRISPR-MA1-089.png
EIF4E (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-WB analysis of K562 whole cell lysate using the EIF4E specific antibody, MA1-089. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the EIF4E-specific antibody, MA1-089. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
- Reviewer comment
- The target is not detected in input which could be normal depending on the antibody sensitivity. The detected band is ~5kDa higher than expected protein size but within 20% thus compliant according to standards.
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U41HG009889