ENCAB861ENQ

Antibody against Homo sapiens H3K36me3

Homo sapiens
any cell type or tissue
characterized to standards
Status
released
Source (vendor)
Diagenode
Product ID
C15410192
Lot ID
A1845P
Host
rabbit
Clonality
polyclonal
Aliases
bradley-bernstein:PchAb 1374

Characterizations

H3K36me3 (Homo sapiens)
HeLa-S3
Method: immunoblot
compliant
Caption
We performed a WB using 0.7ug unmodified recombinant Histone H3 (Millipore 14-494) and 10ug of K562 cell Lysate and 20ug of HeLa nuclear extract. Lysates were resolved by electrophoresis on a 4-12% bis-tris gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with Superblock and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Band of expected size (~17kDa) visualized in HeLa nuclear extract and cannot be detected in unmodified recombinant Histone H3. Antibody seems to be specific since no reactivity with non histone proteins or unmodified histones was detected.
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
H3K36me3 (Homo sapiens)
Method: dot blot assay
Attachment from submitter
compliant
Caption
We performed a DB analysis using the large set of modified peptides provided in the Active Motif MODified Histone Peptide Array, catalog #13005. The only peptide recognized by the H3K36me3 antibody was the correct one.
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad