ENCAB845ARK

Antibody against Homo sapiens H3K4me1

Homo sapiens
any cell type or tissue
characterized to standards
Status
released
Source (vendor)
Diagenode
Product ID
C15410037-50
Lot ID
A1657-D
Characterized targets
H3K4me1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Aliases
bradley-bernstein:PchAb 1382
External resources

Characterizations

H3K4me1 (Homo sapiens)
K562
Method: immunoblot
compliant
Caption
We performed a WB using 10ug of nuclear lysate from K562 cells. Extract was resolved by electrophoresis on a 4-12% Bis-Tris, 1X MES running buffer iBlot transfer, nitrocellulose, P3 for 8 minutes, Blocked in TBST/ 5% NFDM for 1 hour, Antibodies diluted in TBST/ 5% NFDM, imaged in ChemiDoc.
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
H3K4me1 (Homo sapiens)
Method: dot blot assay
compliant
Caption
We performed a DB analysis using the large set of modified peptides provided in the Active Motif MODified Histone Peptide Array, catalog #13005. The only peptide recognized by the H3K4me1 antibody was the correct one.
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad