ENCAB830JLB

Antibody against Homo sapiens CTCF

Homo sapiens
vagina, right lobe of liver, spleen, sigmoid colon, right atrium auricular region, stomach, coronary artery, lower leg skin, thoracic aorta, subcutaneous adipose tissue, thyroid gland, K562, ovary, tibial nerve, tibial artery, adrenal gland, upper lobe of left lung, Peyer's patch, esophagus muscularis mucosa, breast epithelium, transverse colon, esophagus squamous epithelium, prostate gland, testis, gastroesophageal sphincter, body of pancreas, ascending aorta, HepG2, gastrocnemius medialis, heart left ventricle, omental fat pad, suprapubic skin, uterus
characterized to standards with exemption
Status
released
Source (vendor)
Millipore
Product ID
07-729
Lot ID
2599305
Characterized targets
CTCF (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
serum
Antigen description
KLH-conjugated, synthetic peptide corresponding to amino acids 659-675 (C-TNQPKQNQPTAIIQVED) of human CCCTC-binding factor (CTCF) with a N-terminal cysteine added for conjugation purposes.
Aliases
michael-snyder:AS-1647
External resources

Characterizations

CTCF (Homo sapiens)
sigmoid colonstomachtransverse colonsubcutaneous adipose tissuebody of pancreasbreast epitheliumsuprapubic skinspleengastroesophageal sphincteromental fat padlower leg skinuterusthoracic aortaadrenal glandthyroid glandgastrocnemius medialisprostate glandovaryright lobe of livertestistibial nerveupper lobe of left lungesophagus squamous epitheliumesophagus muscularis mucosaascending aortaPeyer's patchcoronary arteryheart left ventriclevaginaright atrium auricular regiontibial artery
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Primary characterizations in each human tissue is not required, given the compliant characterizations in K562 and HepG2 as permitted by section a of page 4 of the May 2016 ENCODE antibody characterization standards document.
Submitter comment
The tissues from GTex are limited and cannot be spared for antibody characterizations.
Reviewer comment
Tissues from EN-tex can be exempted from characterization with compliant characterizations in any two cell types/tissues.
Submitted by
Esther Chan
Lab
Michael Snyder, Stanford
CTCF (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment from submitter
exempt from standards
Caption
Primary characterizations in each human tissue is not required, given the compliant characterizations in K562 and HepG2 as permitted by section a of page 4 of the May 2016 ENCODE antibody characterization standards document. As it is also a new lot of a previously characterized antibody (ENCAB000AXU), if the banding patterns in the primary characterization of the new lot is consistent with that of the old, then a secondary characterization should not be needed.
Submitter comment
This is a new lot of a previously characterized antibody ENCAB000AXU, we think no further characterization is necessary beyond the primary if the banding pattern is consistent with the old.
Reviewer comment
Banding pattern in HepG2 and K562 is consistent with that of the primary characterizations associated with old lot ENCAB000AXU.
Submitted by
Esther Chan
Lab
Michael Snyder, Stanford
CTCF (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody 07-729. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 120
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
CTCF (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody 07-729. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 120
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
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