1. Antibodies
  2. Homo sapiens
  3. NCOR1

ENCAB805KAO

Alternate accessions: ENCAB000AIY, ENCAB035BQS

Antibody against Homo sapiens NCOR1

Homo sapiens
K562
characterized to standards
Homo sapiens
HepG2
characterized to standards with exemption
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-145A
Lot ID
1
Characterized targets
NCOR1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1267, bradley-bernstein:PchAb 163

Characterizations

NCOR1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A301-145A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
NCOR1 (Homo sapiens)
Method: ChIP-string comparison
Attachment PDF Icon
not reviewed
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
NCOR1 (Homo sapiens)
HepG2
Method: immunoblot
Attachment from submitter
Attachment from submitters
exempt from standards
Submitter comment
This is being reviewed by ENCODE2 standards.
Reviewer comment
This experiment was started in ENCODE2 and finished in ENCODE3, the lab argues that ENCODE2 standards should apply.
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
NCOR1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A301-145A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 270.210.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NCOR1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A301-145A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford