1. Antibodies
  2. Homo sapiens
  3. IKZF2

ENCAB794MKC

Antibody against Homo sapiens IKZF2

Homo sapiens
GM12878
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-618A
Lot ID
1
Characterized targets
IKZF2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1603
External resources

Characterizations

IKZF2 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody A303-618A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 59.574
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
IKZF2 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A303-618A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 59.574
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
IKZF2 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody A303-618A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 59.574
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
IKZF2 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from GM12878 nuclear cell lysates using the antibody A303-618A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
In Band A, DSG1, KIFC1 and JUP are not TFs. In Band B, KAP0 is not TF.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
IKZF2 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line GM12878 using the antibody A303-618A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 59.574.
Submitter comment
Multiple immunoreactive bands. We will analyze them all by mass spec in accordance to the ENCODE standards.
Reviewer comment
Multiple immunoreactive bands but analysis by mass spec showed the target TF to be detected in both marked bands.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford