1. Antibodies
  2. Homo sapiens
  3. MATR3

ENCAB789SRM

Antibody against Homo sapiens MATR3

Homo sapiens
K562, HepG2
characterized to standards
Status
released
Source (vendor)
Abcam
Product ID
ab151714
Lot ID
GR108745-9
Characterized targets
MATR3 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Purification
tissue culture supernatant
Isotype
IgG
Antigen description
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Matrin 3 aa 750 to the C-terminus.
Antigen sequence
The exact sequence is proprietary.
External resources

Characterizations

MATR3 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Representative image of immunoprecipitation performed on whole cell extracts from the HepG2 cell line using the MATR3 specific antibody ab151714. Lane 1: Input from IP using control IgG. Lane 2: Immunoprecipitated material using control IgG. Lane 3: Input from IP using MATR3 antibody. Lane 4: Immunoprecipitated material using MATR3 antibody. Outlined regions were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 94.62kDa.
Reviewer comment
Duplicate blot image as previously submitted IP
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
MATR3 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using MATR3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lanes 4 is 10% IP enrichment using rabbit polyclonal MATR3 antibody (lanes under 'MATR3').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
MATR3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Representative image of immunoprecipitation performed on whole cell extracts from the K562 cell line using the MATR3-specific antibody ab151714. Lane 1: Input from IP using control IgG. Lane 2: Immunoprecipitated material using control IgG. Lane 3: Input from IP using MATR3 antibody. Lane 4: Immunoprecipitated material using MATR3 antibody. Outlined regions were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 94.62 kDa.
Reviewer comment
Duplicate blot image as previously submitted IP
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
MATR3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using MATR3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lanes 4 and 5 are 10% IP enrichment of biological replicates using rabbit polyclonal MATR3 antibody (lanes under 'MATR3').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
MATR3 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Protein was immunoprecipitated from HepG2 whole cell lysates using the antibody ab151714, loaded on a 4-12% NuPAGE Bis-Tris gel, and separated via electrophoresis. Using a reference western blot done in parallel, gel pieces corresponding to the sections indicated were excised and submitted for analysis by the UCSD Biomolecular and Proteomics Mass Spectrometry Facility.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
Download
 MATR3 HepG2.pdf
MATR3 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
not reviewed
Caption
IP followed by mass spectrometry. Protein was immunoprecipitated from K562 whole cell lysates using the antibody ab151714, loaded on a 4-12% NuPAGE Bis-Tris gel, and separated via electrophoresis. Using a reference western blot done in parallel, gel pieces corresponding to the sections indicated were excised and submitted for analysis by the UCSD Biomolecular and Proteomics Mass Spectrometry Facility.
Reviewer comment
Not sure how to review, the target factor is top ranked by fold enrichment over IgG but a different factor (ZC3H11A) is second ranked by peptide count but no peptides were detected in the IgG control. However, given there is a passing IP-MS in HepG2, review of this secondary is not required.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
Download
 MATR3 K562.pdf