1. Antibodies
  2. Homo sapiens
  3. DDX1

ENCAB741VRO

Antibody against Homo sapiens DDX1

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Thermo Fisher
Product ID
703141
Lot ID
None
Characterized targets
DDX1 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Purification
Protein A
Isotype
IgG
Antigen description
Protein corresponding to human DDX1 [aa72-aa283]

Characterizations

DDX1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following shRNA against DDX1 in K562 whole cell lysate using DDX1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against DDX1. DDX1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
DDX1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-WB analysis of K562 whole cell lysate using the DDX1 specific antibody, 703141. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the DDX1-specific antibody, 703141. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
Reviewer comment
Eric Mendenhall approved this.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD