ENCAB719MQZ

Antibody against Homo sapiens CTCF

Homo sapiens
HeLa-S3
partially characterized
Status
released
Source (vendor)
Diagenode
Product ID
C15410210
Lot ID
A2359-00234P
Characterized targets
CTCF (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Aliases
bradley-bernstein:PchAb 1378
External resources

Characterizations

CTCF (Homo sapiens)
HeLa-S3
Method: immunoblot
Attachment from submitter
compliant
Caption
0.7 ug unmodified recombinant histone H3 (Millipore 14-494) and nuclear extract from 20 ug HeLa cells were resolved by electrophoresis on a 4-12% acrylamide gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system using program 3. Membrane was blocked for an hou rat room temperature, with 5% BSA in TBS-Tween, and blotted with primary antibody using the dilution stated in the figure, over night at 4C. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) and a ChemiDoc camera (BioRad). Panel A: anti Histone H3 (Abcam ab1791), band of expected size (~17kDa) visualized in unmodified recombinant histone H3, demonstrating the integrity and migration of the recombinant protein. Panel B: Band of expected size (~130kDa) visualized in Nuclear lysates, and cannot be detected in unmodified recombinant Histone H3. Antibody seems to be specific since there is minimal reactivity with nonhistone proteins, and no reactivity with unmodified recombinant histone (loaded in Lane 2 of panel B)
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad