ENCAB700WCJ
Alternate accession: ENCAB906OVC
Antibody against Mus musculus POLR2AphosphoS5
Mus musculus
CH12.LX
partially characterized
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab5408
- Lot ID
- GR106949-6
- Characterized targets
- POLR2AphosphoS5 (Mus musculus)
- Host
- mouse
- Clonality
- monoclonal
- Antigen description
- clone 4H8
- External resources
Characterizations
POLR2AphosphoS5 (Mus musculus)
CH12.LX
Method: immunoblot
compliant
- Caption
- Western blot analysis of POL2 expression in various mouse cell lines. Whole cell extracts prepared from G1E, G1E-ER4+E2, MEL, and CH12 cells were separated by SDS-PAGE and transferred to PVDF using standard immunoblotting methods. POL2 expression was detected using mouse anti-POL24H81 (ab5408, 1:1000) followed by incubation with anti-mouse secondary antibody (1:5000) and detected by ECL Plus (Amersham Biosciences). As expected, a single band of approximately 260 kDa was detected (predicted molecular weight: 217 kDa) in all cell lines tested.
- Submitter comment
- This antibody lot is the same clone as another antibody lot in the system.
- Submitted by
- Cheryl Keller
- Lab
- Ross Hardison, PennState
- Grant
- RC2HG005573