ENCAB697PTS
Antibody against Homo sapiens EIF4B
Homo sapiens
HepG2, K562
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A301-767A
- Lot ID
- 1
- Characterized targets
- EIF4B (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- External resources
Characterizations
EIF4B (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using EIF4B specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal eIF4B Antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- Bethyl_A301-767A_1_EIF4B.png
EIF4B (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against EIF4B in HepG2 whole cell lysate using EIF4B specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4B.EIF4B protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889
- Download
- EIF4B-HEPG2-CRISPR.png
EIF4B (Homo sapiens)
Method: knockdown or knockout
not compliant
- Caption
- Western blot following shRNA against EIF4B in k562 whole cell lysate using EIF4B specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF4B. EIF4B protein appears as the green band, Tubulin serves as a control and appears in red.
- Reviewer comment
- Needs a 50% reduction in signal
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- EIF4B-k562.png
EIF4B (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using EIF4B specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF4B antibody (lanes under 'EIF4B').
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- HepG2_Bethyl_A301-767A_1_EIF4B.png
EIF4B (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using EIF4B specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF4B antibody (lanes under 'EIF4B').
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- K562_Bethyl_A301-767A_1_EIF4B.png