1. Antibodies
  2. Homo sapiens
  3. NIP7

ENCAB696XOD

Antibody against Homo sapiens NIP7

Homo sapiens
HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-974A
Lot ID
1
Characterized targets
NIP7 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
External resources

Characterizations

NIP7 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
IP-WB analysis of K562 whole cell lysate using NIP7 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal anti-NIP7. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
NIP7 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Representative image of immunoprecipitation performed on whole cell extracts from the HepG2 cell line using the NIP7-specific antibody A303-974A. Lane 1: Input from IP using control IgG. Lane 2: Immunoprecipitated material using control IgG. Lane 3: Input from IP using NIP7 antibody. Lane 4: Immunoprecipitated material using NIP7 antibody. Outlined regions were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 20.46 kDa.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
NIP7 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using NIP7 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NIP7 antibody (lanes under 'NIP7').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
NIP7 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Protein was immunoprecipitated from HepG2 whole cell lysates using the antibody A303-974A, loaded on a 4-12% NuPAGE Bis-Tris gel, and separated via electrophoresis. Using a reference western blot done in parallel, gel pieces corresponding to the sections indicated were excised and submitted for analysis by the UCSD Biomolecular and Proteomics Mass Spectrometry Facility.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
Download
 NIP7 HepG2.pdf
NIP7 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against NIP7 in HepG2 whole cell lysate using NIP7 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NIP7.NIP7 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
NIP7 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against NIP7 in K562 whole cell lysate using NIP7 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NIP7.NIP7 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn