1. Antibodies
  2. Homo sapiens
  3. NIPBL

ENCAB695MXG

Antibody against Homo sapiens NIPBL

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Kyoko Yokomori
Product ID
KY_SCC2C_01
Lot ID
93015
Characterized targets
NIPBL (Homo sapiens)
Host
rabbit
Isotype
IgG

Characterizations

NIPBL (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Representative image of immunoprecipitation performed on whole cell extracts from the K562 cell line using the NIPBL-specific antibody KY_SCC2C_01. Lane 1: Input from IP using control IgG. Lane 2: Immunoprecipitated material using control IgG. Lane 3: Input from IP using NIPBL antibody. Lane 4: Immunoprecipitated material using NIPBL antibody. Outlined regions were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 316 kDa.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
NIPBL (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using NIPBL specific antibody. Samples were run on a 3-8% Tris-Acetate with Spectra High Range Ladder. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NIPBL antibody (lanes under 'NIPBL').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
NIPBL (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Protein was immunoprecipitated from K562 whole cell lysates using the antibody KY_SCC2C_01, loaded on a NuPAGE 3-8% Tris-Acetate gel, and separated via electrophoresis. Using a reference western blot done in parallel, gel pieces corresponding to the sections indicated were excised and submitted for analysis by the UCSD Biomolecular and Proteomics Mass Spectrometry Facility.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
Download
 NIPBL K562.pdf