ENCAB686SDS
Antibody against Homo sapiens RYBP
Homo sapiens
K562
characterized to standards
- Status
- released
- Source (vendor)
- Thermo Fisher
- Product ID
- PA5-19943
- Lot ID
- UCF2796583
- Characterized targets
- RYBP (Homo sapiens)
- Host
- rabbit
Characterizations
RYBP (Homo sapiens)
compliant
- Caption
- Western blot following CRISPR against RYBP in HepG2 whole cell lysate using RYBP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RYBP. RYBP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889
- Download
- RYBP-HEPG2-CRISPR-PA5-19943.png
RYBP (Homo sapiens)
K562
compliant
- Caption
- IP-WB analysis of K562 whole cell lysate using the RYBP specific antibody, PA5-19943. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RYBP-specific antibody, PA5-19943. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
- Submitter comment
- Immunoprecipitation lane (3) exhibits a large deviation from the expected protein size, which we believe is due to performing the characterization using the Jess Western Blotting system. This system has a documented tendency for some proteins to run differently than with more traditional methods due to their compositions and interactions with the electrophoretic components. The image also closely resembles the example image provided by the vendor.
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005