1. Antibodies
  2. Homo sapiens
  3. MITF

ENCAB681JHP

Antibody against Homo sapiens MITF

Homo sapiens
K562
partially characterized
Homo sapiens
at least one cell type or tissue
not pursued
Status
released
Source (vendor)
Active Motif
Product ID
39789
Lot ID
11313002
Characterized targets
MITF (Homo sapiens)
Host
mouse
Clonality
monoclonal
Purification
Protein G
Aliases
michael-snyder:AS-819
External resources

Characterizations

MITF (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody 39789. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
IgG appears not different from the IP
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
MITF (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody 39789. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 58.795
Reviewer comment
Band is not clearly absent from the IgG control lane
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 K562-MITF.jpg
MITF (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody 39789. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 58.795.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 MITF.jpg
MITF (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
K562 whole cell lysate was immunoprecipitated using the primary antibody (Active Motif; 39789). The IP fraction was loaded on a 12% Bio-Rad TGX gel and separated with the Bio-Rad Tetra Cell system. A gel fragment (rectangle outline) corresponding to the band indicated on the Coomassie Blue stained gel image was excised and sent to the University of Alabama at Birmingham Cancer Center Mass Spectrometry/Proteomics Shared Facility.
Submitter comment
This image is to show which bands are used for the mass spec
Submitted by
Mark Mackiewicz
Lab
Richard Myers, HAIB