ENCAB615WUN

Antibody against Aequorea victoria eGFP

Homo sapiens
HEK293
characterized to standards
Status
released
Source (vendor)
Abcam
Lot ID
GR194813-1
Host
rabbit
Clonality
polyclonal
Purification
antiserum
Isotype
IgG
Antigen description
Recombinant full length protein corresponding to GFP
Aliases
michael-snyder:AS-1667
External resources

Characterizations

eGFP-ZSCAN26 (Homo sapiens)
Method: motif enrichment
compliant
Caption
The motif for target eGFP-ZSCAN26 is represented by the attached position weight matrix (PWM) derived from ENCFF957YKG. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations, as well as an enrichment rank. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.7952650714 Global enrichment Z-score: 6.94561508003 Positional bias Z-score: 3.06652257649 Peak rank bias Z-score: 4.54167728324 Enrichment rank: 1.0
Submitted by
Jessika Adrian
Lab
Michael Snyder, Stanford
eGFP (Aequorea victoria)
HEK293
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293 using the antibody ab-290 (Lot GR194813-1). The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 28.8.
Reviewer comment
While the western blot was passed strictly on the account that the marked signal comprises of >=50% of the total immunoreactive signal in the lane, the presence of multiple bands is not ideal. The lab will try to repeat the characterization on a different eGFP-tagged HEK293 sample that has not already been crosslinked first like this eGFP-SP6 one.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford