1. Antibodies
  2. Homo sapiens
  3. RACK1

ENCAB611SPG

Antibody against Homo sapiens GNB2L1

Homo sapiens
HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A302-545A
Lot ID
1
Characterized targets
GNB2L1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
External resources

Characterizations

GNB2L1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
IP-WB analysis of K562 whole cell lysate using GNB2L1 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal anti-RACK1. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
GNB2L1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following shRNA against GNB2L1 in K562 and HepG2 whole cell lysate using GNB2L1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against GNB2L1. Lanes 5-8 follow the same pattern, but in HepG2. GNB2L1 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitter comment
K562 shows sufficient knockdown but HepG2 is not at the 50% band intensity
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
GNB2L1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using GNB2L1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GNB2L1 antibody (lanes under 'GNB2L1').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD