ENCAB610PKS
Antibody against Homo sapiens NSUN2
Homo sapiens
K562, HepG2
characterized to standards
- Status
- released
- Source (vendor)
- Thermo Fisher
- Product ID
- 711068
- Lot ID
- SA245649
- Characterized targets
- NSUN2 (Homo sapiens)
- Host
- rabbit
- Purification
- Protein A
- Isotype
- IgG
- Antigen description
- Protein corresponding to Human NSUN2 (350-600)
- External resources
Characterizations
NSUN2 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against NSUN2 in K562 whole cell lysate using NSUN2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against NSUN2. NSUN2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889
- Download
- NSUN2-K562.png
NSUN2 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-WB analysis of K562 whole cell lysate using the NSUN2 specific antibody, 711068. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the NSUN2-specific antibody, 711068. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
- Submitter comment
- Bands at ~55 and ~28kDa in lanes 2 and 4 correspond to the heavy and light chains of the antibody used for the immunoprecipitation.
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U41HG009889
NSUN2 (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-WB analysis of HepG2 whole cell lysate using the NSUN2 specific antibody, 711068. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment using a normal IgG antibody, respectively. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the NSUN2-specific antibody, 711068. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005