ENCAB588PCU

Antibody against Homo sapiens DEK

Homo sapiens
HeLa-S3, K562
characterized to standards
Status
released
Source (vendor)
Novus
Product ID
NB100-61057
Lot ID
A1
Host
rabbit
Aliases
michael-snyder:DEK_NB100-61057_A1
External resources

Characterizations

DEK (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation of DEK from K562 cells using NB100-61057. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with NB100-61057. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. The expected band size is 43 kDa.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
DEK (Homo sapiens)
HeLa-S3
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HeLa-S3, using the antibody NB100-61057. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 39.354
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
DEK (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using NB100-61057, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by Colloidial Coomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford