ENCAB584CZQ

Antibody against Homo sapiens REST

Homo sapiens
HepG2
characterized to standards with exemption
Homo sapiens
K562, endothelial cell of umbilical vein, GM12878
not characterized to standards
Status
released
Source (vendor)
Millipore
Product ID
17-641
Lot ID
NG1875688
Host
rabbit
Clonality
polyclonal
Antigen description
RE1-silencing transcription factor
Aliases
bradley-bernstein:PchAb 448
External resources

Characterizations

REST (Homo sapiens)
Method: ChIP-seq comparison
exempt from standards
Caption
REST is a sequence specific binding factor. As such, the validation standard for REST antibodies is distinct from the chromatin regulator validation standard under which the remainder of our antibody validation dossiers are being constructed. The present document does not intend to substitute for the full computational analysis for the enrichment of the recognition sequence for REST in peaks discovered using ChIP-seq. The present document will merely illustrate the tracks we have obtained using two distinct lots of a particular antibody, and will present a single illustration of the occurrence of a perfect occurrence of the REST recognition sequence in tight proximity to a ChIP-seq peak, using a particular locus [CHRNB2 (cholinergic receptor nicotinic beta 2 subunit)] identified in the classic literature on the genome-wide occurrence of the recognition motif of the neuron-restrictive silencing factor (REST). Many thousands of other such illustrations could also be presented.
Submitter comment
This was cleared by the antibody committee
Reviewer comment
This was cleared by the antibody committee as not ideal but releasable
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
REST (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from K562(10ug), GM12878 (10ug),HUVEC (10ug), HepG2 (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a stronger band of expected size was detected (~120kDa).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad