1. Antibodies
  2. Homo sapiens
  3. EEF2

ENCAB559BYO

Antibody against Homo sapiens EEF2

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Thermo Fisher
Product ID
711842
Lot ID
1931156
Characterized targets
EEF2 (Homo sapiens)
Host
rabbit
Purification
Protein A
Isotype
IgG
Antigen description
Protein corresponding to human EEF2 (aa241-aa340)
External resources

Characterizations

EEF2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following shRNA against EEF2 in K562 whole cell lysate using EEF2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against EEF2. EEF2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
EEF2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-WB analysis of K562 whole cell lysate using the EEF2 specific antibody, 711842. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the EEF2-specific antibody, 711842. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
Reviewer comment
Lane was corrected to 4 (2022/08/08).
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD