1. Antibodies
  2. Homo sapiens
  3. NUFIP1

ENCAB558VPN

Antibody against Homo sapiens NUFIP1

Homo sapiens
K562
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-148A
Lot ID
1
Characterized targets
NUFIP1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1585
External resources

Characterizations

NUFIP1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A303-148A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 56.300
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NUFIP1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A303-148A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 56.3 kDa
Submitter comment
Lower bad running between 37 and 50 kDa has a faint band in IgG lane.
Reviewer comment
Multiple immunoreactive bands present at about equal intensities. However, the submitter has argued for the lower sized band (~50kb) to be that from the primary antibody's denatured heavy chain, also seen faintly in the IgG lane.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford