1. Antibodies
  2. Homo sapiens
  3. CBX7

ENCAB557PEK

Antibody against Homo sapiens CBX7

Homo sapiens
K562, HepG2, GM12878, SK-N-SH, A549, H1
partially characterized
Status
released
Source (vendor)
Abcam
Product ID
ab91431
Lot ID
GR38055-4
Characterized targets
CBX7 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Antigen description
RING1 interacting PRC1 component
Aliases
bradley-bernstein:PchAb 1226
External resources

Characterizations

CBX7 (Homo sapiens)
A549H1
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Nuclear lysates from A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected above the expected size (28kDa).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
CBX7 (Homo sapiens)
K562HepG2GM12878SK-N-SH
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (7ug), GM12878 (7ug), SK-N-SH (6ug), were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using the iblot system. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min.the strongest band was detected around the expected size (~30kDa).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad